mouse anti myogenic differentiation antigen myod  (Santa Cruz Biotechnology)

Journal: Journal of Advanced Research

Article Title: Versatile platforms of mussel-inspired agarose scaffold for cell cultured meat

doi: 10.1016/j.jare.2025.01.024

Figure Lengend Snippet: Proliferation of C2C12 cells on AG/PDA gel scaffold. a) Representative images of C2C12 cells stained with EdU incorporation assay on AG/PDA gel scaffolds, b) EdU positive cells of C2C12 cells were evaluated (n = 6). The PDA coating has optical absorption properties, color codes: blue: nuclei; red: EdU, multicolor: merge, bars: 100 μm. c) Western blot analysis of MYOD and GADPH protein levels in the proliferative and differentiation phases (n = 3). d ) Quantification of MYOD expression normalized to GAPDH from western blot gel (n = 3). Data presented as mean ± SD (* p < 0.05, ** p < 0.01 and *** p < 0.001, ns: not significant).

Article Snippet: After sealing the membranes with 5 % bovine serum albumin buffer for 2 h at room temperature, the membranes were incubated at 4 °C overnight with primary antibodies ((rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, ab181602, 1:10000), mouse anti- myogenic differentiation antigen (MyoD) (Santa Cruz Biotechnology, sc-377460, 1:500), primary MYHC antibody (1:500), then secondary antibodies (horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Beyotime, A208,1:1000), HRP-conjugated goat anti-mouse antibody (Beyotime, A216, 1:1000) was incubated for 1 h at room temperature.

Techniques: Staining, Western Blot, Expressing

Journal: Journal of Advanced Research

Article Title: Versatile platforms of mussel-inspired agarose scaffold for cell cultured meat

doi: 10.1016/j.jare.2025.01.024

Figure Lengend Snippet: Myogenesis of PSMSCs on AG/PDA gel scaffold. a) Representative photographic images of PMSCs were first adhesion after inoculation on AG/PDA gel scaffolds for two hours. b) Adhesion ratio of PSMSCs on AG/PDA gel scaffolds (n = 6). c) Representative images of PSMSCs stained with EdU incorporation assay on AG/PDA gel scaffolds. d) EdU positive cells of PSMSCs were evaluated (n = 6). The PDA coating has optical absorption properties, color codes: blue: nuclei; red: EdU, multicolor: merge, bars: 50 μm. e) the metabolic activity of PSMSCs with alma blue cell viability assay on AG/PDA gel scaffolds (n = 6). f) Representative images of immunofluorescent stained PSMSCs and myotubes after 5 days of differentiation on AG/PDA gel scaffolds. Color codes: blue: nuclei, red: MYHC, green: phalloidin, multicolor: merge, bars: 50 μm. g) Western blot analysis of MYHC, MYOD, and GAPDH protein levels in the proliferative and differentiation phases (n = 3). h) Quantification of MYHC and MYOD expression normalized to GAPDH from western blot gel (n = 3). Data presented as mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001, ns: not significant).

Article Snippet: After sealing the membranes with 5 % bovine serum albumin buffer for 2 h at room temperature, the membranes were incubated at 4 °C overnight with primary antibodies ((rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, ab181602, 1:10000), mouse anti- myogenic differentiation antigen (MyoD) (Santa Cruz Biotechnology, sc-377460, 1:500), primary MYHC antibody (1:500), then secondary antibodies (horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Beyotime, A208,1:1000), HRP-conjugated goat anti-mouse antibody (Beyotime, A216, 1:1000) was incubated for 1 h at room temperature.

Techniques: Staining, Activity Assay, Viability Assay, Western Blot, Expressing

Journal: Journal of Advanced Research

Article Title: Versatile platforms of mussel-inspired agarose scaffold for cell cultured meat

doi: 10.1016/j.jare.2025.01.024

Figure Lengend Snippet: Myogenesis of PSMSCs on AG/PDA film scaffold. a) Schematic of the production process of film scaffolds. b) Representative SEM images of the PDA particles on film scaffold, bar: 1 μm. c) Analysis of the surface hydrophilic angle on film scaffolds (n = 3). d) Representative images of surface hydrophilic angles of film scaffolds and Water absorption swelling rate of film scaffolds (n = 3). e) Representative SEM images of the AG/PDA-0.2 film scaffold surface with and without PSMSCs adhesion, bar: 1 μm. f) the metabolic activity of PSMSCs with alma blue cell viability assay on AG/PDA film scaffolds (n = 6). g) Representative 3D reconstruction images of immunofluorescent stained PSMSCs and myotubes after 5 days differentiation on AG/PDA film scaffolds. Color codes: blue: nuclei, red: MYHC, green: phalloidin, multicolor: merge, bars: 150 μm. h) Western blot analysis of MYHC, MYOD, and GAPDH protein levels in the proliferative and differentiation phases (n = 3). i) Quantification of MYHC and MYOD expression normalized to GAPDH from western blot gel (n = 3). Data presented as mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001, ns: not significant).

Article Snippet: After sealing the membranes with 5 % bovine serum albumin buffer for 2 h at room temperature, the membranes were incubated at 4 °C overnight with primary antibodies ((rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, ab181602, 1:10000), mouse anti- myogenic differentiation antigen (MyoD) (Santa Cruz Biotechnology, sc-377460, 1:500), primary MYHC antibody (1:500), then secondary antibodies (horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Beyotime, A208,1:1000), HRP-conjugated goat anti-mouse antibody (Beyotime, A216, 1:1000) was incubated for 1 h at room temperature.

Techniques: Activity Assay, Viability Assay, Staining, Western Blot, Expressing